The aim of this study was to investigate the effectiveness of humor skills training on psychological hardiness and psychological helplessness of mothers with children with autism spectrum disorder. According to the objectives and hypotheses, the present study method is a semi-experimental type (pre-test-post-test design with control group). The statistical population of this study included all mothers of children with autism spectrum disorder who referred to autistic pediatric clinics in the first half of 2020 in Ardabil. Among mothers of children with autism, 40 mothers were selected by available method and randomly assigned to two groups of 20 patients and at least 15 samples were suggested in experimental research. The data collection tool in this study is a questionnaire that standard questionnaires of psychological hardiness, learned helplessness have been used. Statistical indicators used include calculation of frequency, mean and standard deviation. Multivariate analysis of variance (MANOVA) was used to test the research hypotheses. Data analysis was performed using SPSS statistical software, 18th edition. The results showed that the psychological hardiness of mothers with children with autism spectrum disorder who received humor skills training was higher than mothers who did not receive this training. The dimensions of psychological hardiness variable (commitment, control and struggle) of mothers who received humor skills training are more than mothers who did not receive this training and there is a significant difference between the amount of learned helplessness of mothers of children with autism spectrum in the experimental and control group, so that the learned helplessness of mothers who received humor skills is less.